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pHY10
pHY10
規(guī)格:
價格:詢價
編號:B212580
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHY10
商品貨號
B212580
Designations
pHY10
Species
Homo sapiens, human
培養(yǎng)基
Applications
TaqI and StuI digests seem to reveal polymorphisms but they are too dependent on hybridization conditions for definitive segregation studies.
Vector
Construct size (kb): 9.5600004196167
Insert
DNA: genomic
Insert lengths(kb): 3.563999891281128
Tissue: fibroblast
Gene product: DNA Segment, repetitive [DYZ1]
Insert Size (kb)
3.564
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 3.55; BamHI--9.5; HindIII--9.5; AvaI--9.5; PvuII--6.8, 2.7.
Contains 64 TaqI sites, 74 HinfI sites, 5 RsaI sites at 507, 822, 2610, 3095, 3176; 2 Sau3AI sites at 575, 2304; 1 HaeIII site at 3526, 1 ClaI site at 1067; and no MspI or SphI sites.
Sequence is a tandem repeat of pentanucleotides; 229/713 were TTCCA and 298/713 were single nucleotide substitutions of that: 58-TTCGA, 33-TTCCT, 31-GTCCA, 27-TTCTA, 23-TTCAA, 22-TTGCA, 20-TTTCA.
A 3.55 kb band is revealed in EcoRI, HaeIII, and StuI digests. TaqI and StuI digests seem to reveal polymorphisms but they are too dependent on hybridization conditions for definitive segregation studies.
References

Nakahori Y, et al. A human Y-chromosome specific repeated DNA family (DYZ1) consists of a tandem array of pentanucleotides. Nucleic Acids Res. 14: 7569-7580, 1986. PubMed: 3774538

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