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HuHsp90-#2
HuHsp90-#2
規格:
價格:
編號:B213203
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HuHsp90-#2
商品貨號
B213203
Designations
HuHsp90-#2
GenBank Number

M16660

培養基
Species
Homo sapiens, human
Depositors
NF Rebbe
Vector
Construct size (kb): 5.699999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS-
Intact vector size: 2.964
Type of vector: phagemid
Vector end: SalI
Vector end: SalI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HSPCB
Other gene symbols: HSPC2
Gene name: heat shock 90kD protein 1, beta
Contains complete coding sequence?: Y
Localization: 6 p12
Tissue: fetal liver
Type of DNA: cDNA
Insert end: SalI
Insert end: SalI
Insert size (kb): 2.739
Cross references: DNA Seq. Acc.: M16660
Nucleotides 13-2524 of the insert correspond to
nucleotides 32-2543 of M16660.
Insert lengths(kb): 2.739000082015991
Tissue: fetal liver
Gene product: heat shock 90kD protein 1, beta [HSPC2]
Insert Size (kb)
2.739
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.9, 0.7; HindIII--4.4, 1.4; KpnI--5.0, 0.64; PvuII--2.5, 1.7, 1.5; SalI--3.0, 2.7.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.08; BglI--0.11; EcoRI--0.97, 1.05; HindIII--1.38; KpnI--0.60; PstI--2.17, 2.30; PvuII--1.16; SacI--1.30; XhoI--2.20, 2.31.
The SalI insert contains the complete coding sequence and a poly(A) tail followed by a cloning artifact of approximately 180 bp.
The genomic sequence is available in GenBank/EMBL/DDBJ accession J04988.
References

Rebbe NF, et al. Nucleotide sequence and regulation of a human 90-kDa heat shock protein gene. J. Biol. Chem. 264: 15006-15011, 1989. PubMed: 2768249

Rebbe NF, et al. Nucleotide sequence of a cDNA for a member of the human 90-kDa heat-shock protein family. Gene 53: 235-245, 1987. PubMed: 3301534

Neil F Rebbe, personal communication

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