91麻豆精品国产91久久久更新资源速度超快,夜色成人网,欧美亚洲综合在线

pTR-UF-11 plasmid in <em>E. coli</em>

規格:

價格:詢價

編號:B213352

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

pTR-UF-11 plasmid in E. coli

商品貨號

B213352

Designations

pTR-UF-11 plasmid in E. coli

Depositors

University of Florida

培養基

Biosafety Level

Host

E. coli SURE Cells from Stratagene

Vector Information

Vector size (in kb): 7.2
Vector type: AAV vector plasmid
Vector features: CMV IE enhancer, Chicken β-actin promoter, AAV2 Inverted Terminal Repeats (ITRs), SV40 polyA, bovine growth hormone polyA
Bacterial selection: Ampicillin

Applications

SmaI cuts in both AAV ITRs and can serve as a diagnostic that the ITRs are intact.

Comments

SmaI cuts in both AAV ITRs (these can be unstable in bacteria). When culturing the bacteria containing AAV ITRs, do not culture for more than 16-18 hours at 37°C because deletions may occur especially in stationary cultures. A missing ITR can be tolerated in about 20% of a plasmid prep. SmaI cuts in both AAV ITRs and can serve as a diagnostic that the ITRs are intact. One or the other ITRs may be deleted (and so will the SmaI site) during culturing and cloning. If an ITR is deleted, then the SmaI digests will generate larger molecular weight bands.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37°C

Propagation:
Streak a loopful of culture on an LB + 50 µg/mL ampicillin agar plate. Incubate liquid cultures at 37°C for no more than 16-18 hours. Isolate DNA using standard plasmid preparation procedures. Up to 20% of the plasmid population with one ITR deleted is tolerated

References

Burger C. et al. Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous system. Molecular Therapy : 10(2):302-17, 2004 PubMed: 15294177