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pKBY2

規格:

價格:詢價

編號:B213660

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pKBY2

商品貨號

B213660

Designations

pKBY2

Depositors

Regents of the University of California, WE Timberlake, Regents of the University of California

培養基

U.S. Patent

4,816,405

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Biosafety Level

Host

Distribution host: Escherichia coli HB101

Vector Information

Size (kb): 9.8999996185302730

Vector: pKBY2 (cosmid)

Construction: pBR329, Aspergillus nidulans trpC, pJB8 cos

Marker(s):ampR,cmlR,trpC+

Construct size (kb): 9.899999618530273

Features: marker(s): ampR, cmlR, trpC+
replicon: pMB1
enhancer: none

Applications

shuttle vector

vector for constructing genomic libraries

Comments

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

This is a cosmid vector for isolating genes by complementation of mutations in Aspergillus nidulans. It can also be used for library construction.

A 2.1 kb HindIII/SalI fragment (pJB8) containing lambda cos was ligated to HindIII/SalI-digested pBR329. A 4.1 kb XhoI fragment with wild-type Aspergillus nidulans trpC was ligated into the SalI, and a BamHI linker into the NruI site of pBR329.

The BamHI site was removed from trpC by sodium bisulfite mutagenesis.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 30.0°C

References

Yelton MM, et al. A cosmid for selecting genes by complementation in Aspergillus nidulans: selection of the developmentally regulated yA locus. Proc. Natl. Acad. Sci. USA 82: 834-838, 1985.

Shipped

frozen

Shipping Information

Distributed: freeze-dried

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