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pGAR1 [CMCC 1775]
pGAR1 [CMCC 1775]
規(guī)格:
價格:詢價
編號:B213750
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pGAR1 [CMCC 1775]
商品貨號
B213750
Designations
pGAR1 [CMCC 1775]
Species
Aspergillus awamori Nakazawa
培養(yǎng)基
Depositors
Cetus Corp., A Belt, Cetus Corp.
Vector
Construct size (kb): 7.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pACYC184
Intact vector size: 4.244
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI HindIII BamHI SalI
Polylinker sites:
Construction: p15A, pSC101, Tn9
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): cmlR, tetR
replicon: p15A
Cross references: DNA Seq. Acc.: X06403
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Aspergillus awamori
Gene name: glucoamylase
Contains complete coding sequence?: U
Strain: NRRL 15271
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 3.4
Cross references:
Insert lengths(kb): 3.400000095367432
Gene product: glucoamylase
Insert Size (kb)
3.400
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.4 (intron 1); BglII--1.0 (intron 3); PstI--1.1, 3.0 (exon 4, 3' flanking); BamHI--1.9 (exon 5); SalI--2.6 (3' flanking).
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.4; BamHI--4.1, 3.1; PstI--3.8, 1.8 (doublet).
The insert detects a mRNA of 2.2 kb.
References

Nunberg J, et al. Glucoamylase cDNA. US Patent 4,794,175 dated Dec 27 1988

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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