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pPR969 [NEB687]
pPR969 [NEB687]
規(guī)格:
價格:詢價
編號:B214164
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
pPR969 [NEB687]
商品貨號
B214164
Designations
pPR969 [NEB687]
GenBank Number

M74198

培養(yǎng)基
Species
Thermococcus litoralis Neuner et al.
Depositors
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Vector
Construct size (kb): 10.1
Insert
DNA: genomic
Insert lengths(kb): 3.824
Gene product: DNA polymerase (Vent) [pol]
Insert Size (kb)
3.900
Media
ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--11.5; EcoRI--9.4, 2.0; HindIII--10.75, 0.68.
Constructed by modifying the sequence TTTATG to CATATG to create a NdeI site at the 5' end (nt 288-293), and by removing the intervening sequence spanning nucleotides 1776 and 3398 of the sequence record.
References

Comb DG, et al. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis. US Patent 5,322,785 dated Jun 21 1994

Comb DG, et al. Recombinant thermostable DNA polymerase from archaebacteria. US Patent 5,352,778 dated Oct 4 1994

Perler FB, et al. Intervening sequences in an Archaea DNA polymerase gene. Proc. Natl. Acad. Sci. USA 89: 5577-5581, 1992. PubMed: 1608969

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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