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pKB750

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價格:詢價

編號:B214833

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

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產品名稱

pKB750

商品貨號

B214833

Designations

pKB750

GenBank Number

M10431

培養基

Species

Escherichia coli (Migula) Castellani and Chalmers

Depositors

OmniGene, Inc., KC Backman, OmniGene, Inc.

Applications

produces protein chorismate mutase-P-prephenate dehydrogenase

Vector

Construct size (kb): 7.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749

Insert

DNA: genomic

Insert lengths(kb): 1.379999995231628

Gene product: 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase [aroF]

Target Gene: chorismate mutase-P-prephenate dehydrogenase

Insert Size (kb)

1.380

Media

ATCC^® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/KpnI--4.4, 1.4, 1.2; EcoRV--4.4, 2.6; EcoRI--4.4, 2.6.

Expression from the aroF promoter results in an aroF-pheA fusion protein. Insert contains aa 1-351 of the aroF coding sequence and the complete pheA coding sequence.

The construct contains a weak attenuator sequence at the 5' end of the pheA coding sequence.

Production of phenylalanine is enhanced when the pheA structural gene is introduced as part of a synthetic operon, rather than as a single deregulated structural gene.

Classification

Enterobacteriaceae, Escherichia

References

Backman KC. Method of biosynthesis and cells therefor. US Patent 4,839,286 dated Jun 13 1989

Backman KC. Enzyme deregulation. US Patent 4,753,883 dated Jun 28 1988

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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