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p416 GALL

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編號:B215267

品牌:Mingzhoubio

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產品名稱

p416 GALL

商品貨號

B215267

Designations

p416 GALL

Depositors

M Funk

培養基

Biosafety Level

Host

Distribution host: Escherichia coli HB101 (ATCC 33694)

Vector Information

Size (kb): 5.5539999008178710

DESCRIPTION OF VECTOR:
Intact vector size: 5.554
Type of vector: plasmid
Cloning sites: XbaI SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS416, GALL promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1323
terminator: CYC1, ->, 2005-2265
MCS: XhoI...XbaI, ->, 2265-2328
promoter for expression: GALL, <-, 2329-2759
marker(s): ampR, <-, 4006-4936
centromere: CEN6/ARSH4, ->, 5040-5554

Vector: p416 GALL (plasmid)

Promoters: Promoter for expression GALL

Construction: pRS416, GALL promoter

Marker(s):URA3,ampR

Construct size (kb): 5.553999900817871

Features: marker(s): URA3
marker(s): ampR
promoter for expression: GALL
MCS: XhoI...XbaI
terminator: CYC1
centromere: CEN6/ARSH4

Applications

YX-type (expression) shuttle vector

vector with low copy number

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--5.1, 0.43.

The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose.

The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.

Low copy number shuttle expression vector.

One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736