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pDG1
pDG1
規格:
價格:詢價
編號:B215473
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pDG1
商品貨號
B215473
Designations
pDG1
Depositors
Allelix Biopharmaceuticals, Inc., G Strachan, Allelix Biopharmaceuticals, Inc.
培養基
U.S. Patent
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
1
Host
Distribution host: Escherichia coli HB101
Vector Information
Size (kb): 12.1999998092651400
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Vector: pDG1 (cosmid)
Construction: pHC79, pBB116
Marker(s):ampR,argB
Construct size (kb): 12.19999980926514
Features: marker(s): ampR, argB
replicon: pMB1
Applications
contains sequence ornithine carbamoyltransferase
integrating vector
shuttle vector
vector for constructing genomic libraries
vector permitting positive selection for integration
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--9.4, 1.5, 1.3; EcoRI/BamHI--6.3, 5.9; PstI--10.2, 2.0.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
This is an integrative, positive selection vector conferring arginine prototrophy on an auxotrophic host such as Aspergillus niger 350(-)52 (ATCC 20739) (argB-).
The argB BamHI/EcoRI fragment contains the following sites (separated by approximate kb): BamHI- 0.8- SalI- 0.4- HindIII- 1.4- SalI- 0.1- HindIII- 0.7- SalI- 0.1- HindIII- 0.7- SalI- 0.7- SalI- 0.5- EcoRI.
This was constructed by inserting the 5.0 kb EcoRI/HindIII fragment from pBB116 containing argB into pHC79 cut with EcoRI + HindIII.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Buxton FP, et al. Aspergillus niger transformation system. US Patent 4,885,249 dated Dec 5 1989

Berse B, et al. Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans. Gene 25: 109-117, 1983. PubMed: 6363209

Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
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