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KS+SV0CAT

規格:

價格:詢價

編號:B215985

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

KS+SV0CAT

商品貨號

B215985

Designations

KS+SV0CAT

Depositors

PA Tsonis

培養基

Biosafety Level

Vector Information

Size (kb): 4.9000000953674320

Vector: KS+SV0CAT (plasmid)

Promoters: Promoter T3

Construction: cml from pSVOcat, pBluescript KS+

Marker(s):ampR

Construct size (kb): 4.900000095367432

Features: insert detection: CAT
marker(s): ampR
promoter: T3, T7
replicon: pMB1

Applications

expression vector

promoter-cloning vector

shuttle vector

vector permitting RNA synthesis in vitro

Comments

HindIII-ApaI fragment from pSV2cat contains internal BamHI and EcoRI sites.

Restriction digests of the clone give the following sizes (kb): SacI--4.4; Xba--4.4; BamHI--2.9, 1.6; SmaI--4.4; PstI--4.4; EcoRI--3.9, 0.32; HindIII--4.4.

Mammalian shuttle expression vector for promoter cloning with activity analysis by CAT (chloramphenicol acetyltransferase) assay. Use KS+SV2CAT (ATCC 37609) as a control.

Constructed by inserting the HindIII-ApaI fragment of pSV2cat in HindIII-ApaI digested Bluescript KS+. Contains no SV40 promoter.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Tsonis PA, et al. CAT constructs with convenient sites for cloning and generating deletions. Nucleic Acids Res. 16: 7745, 1988. PubMed: 3166134

Panagiotis A Tsonis, personal communication

Shipped

freeze-dried

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