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lambdaZd31

規(guī)格:

價(jià)格:詢價(jià)

編號(hào):B216575

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

lambdaZd31

商品貨號(hào)

B216575

Designations

lambdaZd31

Depositors

AJ Murphy

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 43.70

Vector: lambdaZd31 (phage, lambda - replacement)

Promoters: Promoter Mo-MLV LTR

Construction: Charon 30, pZIP-neoSV(x)1

Marker(s):G418R,ampR,kanR,neoR

Construct size (kb): 43.70

Features: marker(s): ampR, kanR, neoR, G418R
promoter: Mo-MLV LTR
replicon: lambda
terminator: 3' LTR

Applications

expression vector

lambda vector allowing conversion to plasmid

shuttle vector

vector for constructing cDNA libraries

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--19, 25.

The plasmid insert can be released by homologous recombination between the 2 LTRs after growth at low temperature in Escherichia coli RRI(pRK248), ATCC 47036.

A cell line suitable for transfection by released plasmid is Wgd5, ATCC CRL 1817.

Allows expression of cloned cDNA in mammalian cells; can be used for library transduction.

Contains Mo-MLV LTR for transcription of cDNA and neo; ori of SV40 and pBR322.

References

Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791

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freeze-dried

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