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pCaD4
pCaD4
規(guī)格:
價格:
編號:B216751
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pCaD4
商品貨號
B216751
Designations
pCaD4
Species
Cauliflower mosaic virus
培養(yǎng)基
Depositors
JE Schoelz
Vector
Construct size (kb): 12.39400005340576
Insert
DNA: genomic
Insert lengths(kb): 8.0
Insert Size (kb)
8.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The clone contains the following restriction sites (using the viral numbering scheme from 1 to the SalI site at 4833): BamHI--1926, 2148, 8921; EcoRI--408, 2414, 2474, 8546, 10009, 10681; SacI--3750, 10185; PvuII--4000, 6249, 10681; SalI--4833, 9196.
Restriction digests of the clone give the following sizes (kb): SalI--8.2, 4.4; EcoRI--6.5, 2.5, 2.2, 1.5, 0.5; HindIII--4.3, 4.0, 1.2, 1.0, 0.6 (doublet), 0.5; BamHI--6.8, 6.0; PstI--6.5, 4.4, 2.2.
Known genes have these approximate positions (virus numbering scheme): I: 300-1300; II (aphid helper component): 1300-1800; III: 1800-2200; IV (coat protein):2200-3600; V (reverse transcriptase): 3600-5600; VI (inclusion body protein): 5800-7300.
This strain was isolated from crucifers in Davis, California.
This was constructed by linearizing the full-length CaMV DNA with SalI and inserting it into the SalI site of pBR322.
Classification
Caulimovirus
References

Gardner RC, et al. The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus of M13mp7 shotgun sequencing. Nucleic Acids Res. 9: 2871-2888, 1981. PubMed: 6269062

Schoelz JE, et al. Properties of an unusual strain of cauliflower mosaic virus. Phytopathology 76: 451-454, 1986.

James E Schoelz, personal communication

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