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pCD.2
pCD.2
規格:
價格:詢價
編號:B217553
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCD.2
商品貨號
B217553
Designations
pCD.2
Depositors
PC Zambryski
培養基
Biosafety Level
1
Host
Distribution host: Escherichia coli DH5
Vector Information
Size (kb): 6.2199997901916500
DESCRIPTION OF VECTOR:
Intact vector size: 6.220
Type of vector: plasmid
Cloning sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI
Polylinker sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI
Construction: pRS314
Features (with orientation and position when available):
replicon: ColE1, 1-362
coding sequence: bla, <-, 934-1794
replicon: ARS4, 2066-2442
centromere: CEN6, 2028-2053
marker(s): TRP1, ->, 2976-3650
promoter: PADH, 4437-5165
MCS: NdeI..PstI, ->, 5661-5730
ribosome-binding site: GAL4, ->, 5166-5607
transcription terminator: ADC1 transcriptional terminator (TADH), 5748-5938
Vector: pCD.2 (plasmid)
Promoters: Promoter PADH
Construction: pRS314
Marker(s):crbR
Construct size (kb): 6.21999979019165
Features: marker(s): TRP1
promoter: PADH
replicon: ARS4
replicon: ColE1
MCS: NdeI..PstI
ribosome-binding site: GAL4
transcription terminator: ADC1 transcriptional terminator (TADH)
centromere: CEN6
coding sequence: bla
Applications
cloning vector
shuttle vector
vector component of two-hybrid screening system
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.2; PstI--6.2; HindIII--4.5, 1.7; NdeI--6.2.
Useful in two hybrid screening in yeast. A CEN-based plasmid with the Gal4' DNA binding domain. It has lower copy number than 2 micron based plasmids.
Contains unique rstriction sites (BglII, BclI and BamHI) compatible with 5' GATC overhangs in all three reading frames.
Media
ATCC® Medium 1637: LB medium (ATCC medium 1065) with 100 mcg/ml carbenicillin
Growth Conditions
Temperature: 37.0°C
References

Durfee T, et al. New tools for protein linkage mapping and general two-hybrid screening. Yeast 15: 1761-1768, 1999. PubMed: 10590464

Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
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