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hHAOF9
hHAOF9
規(guī)格:
價格:詢價
編號:B217821
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
hHAOF9
商品貨號
B217821
Designations
hHAOF9
GenBank Number

Z29481

培養(yǎng)基
Species
Homo sapiens, human
Depositors
P Malherbe
Applications
in another host, produces protein 3-hydroxyanthranilate 3,4-dioxygenase
Vector
Construct size (kb): 6.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBC/CMV
Intact vector size: 4.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII
Polylinker sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII
Host range: eukaryotic cells; Escherichia coli
Features (with orientation and position when available):
replicon: pMB1
promoter for expression: CMV, ->
other: rat preproinsulin II (rI2) intron, ->
terminator: SV40 polyadenylation signal, ->
marker(s): ampR
replicon: SV40
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HAO-PEN
Gene name: 3-hydroxyanthranilate 3,4-dioxygenase
Contains complete coding sequence?: Y
Tissue: hepatoma HepG2 cell line (ATCC HB8065)
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.276
Cross references: DNA Seq. Acc.: Z29481
Nucleotides 1-1247 of the insert correspond to
nucleotides 1-1247 of Z29481.
Insert lengths(kb): 1.276000022888184
Tissue: hepatoma HepG2 cell line (ATCC HB8065)
Gene product: 3-hydroxyanthranilate 3,4-dioxygenase [HAO-PEN]
Target Gene: 3-hydroxyanthranilate 3,4-dioxygenase
Insert Size (kb)
1.276
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.7, 1.3; NcoI--4.0, 0.9, 0.5, 0.4, 0.3; PstI--5.4, 0.6.
Insert contains the following restriction sites (approximate kb from the 5' end): NcoI--0.41, 0.91; PstI--0.62, 1.18; PvuII--0.21, 0.53, 0.73; SmaI--0.03; StuI--0.70; XhoI--0.95.
Includes 40 nt of 5' untranslated sequence, the complete coding sequence and 346 nt of 3' untranslated sequence followed by a poly(A) tail
References

Malherbe P, et al. Molecular cloning and functional expression of human 3-hydroxyanthranilic-acid dioxygenase. J. Biol. Chem. 269: 13792-13797, 1994. PubMed: 7514594

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