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pbPKC-alpha21
pbPKC-alpha21
規格:
價格:詢價
編號:B218095
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pbPKC-alpha21
商品貨號
B218095
Designations
pbPKC-alpha21
GenBank Number

M13973

培養基
Species
Bos taurus, cow
Depositors
A Ullrich
Applications
Hybridizes to human sequences and was used to map the human gene.
Vector
Construct size (kb): 3.200000047683716
Insert
DNA: cDNA
Insert lengths(kb): 0.531000018119812
Tissue: brain
Gene product: protein kinase C, alpha [Pkca]
Alleles: b, s
Insert Size (kb)
0.531
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Contains the following restriction sites (with nucleotide position according to the published sequence): BamHI--608, HindIII--645, HindIII--975.
Restriction digests of the clone give the following sizes (kb): BamHI--2.75, 0.45; PstI--3.2; XbaI--3.2; HindIII--2.85, 0.35; EcoRI--2.7, 0.5.
Includes sequences from approximately nt 590 to nt 1120 of the published sequence, encoding the calcium-binding site and the ATP binding site. Coding sequences extend from nt 50 to nt 2066.
Hybridizes to human sequences and was used to map the human gene. Detects human genomic EcoRI fragments of 4.7 and 3.8 kb and HindIII fragments of 4.2, 7.5, and 3.0 kb.
Detects mRNA of 8.1, 3.8, and 3.5 kb in bovine brain and 8.6 kb in rat brain.
References

Buchberg AM, et al. A comprehensive genetic map of murine chromosome 11 reveals extensive linkage conservation between mouse and human. Genetics 122: 153-161, 1989. PubMed: 2567264

Coussens L, et al. Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways. Science 233: 859-866, 1986. PubMed: 3755548

Parker PJ, et al. The complete primary structure of protein kinase C--the major phorbol ester receptor. Science 233: 853-859, 1986. PubMed: 3755547

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