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pMCMP1
pMCMP1
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B219026
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMCMP1
商品貨號(hào)
B219026
Designations
pMCMP1
Species
Homo sapiens, human
培養(yǎng)基
Depositors
RL White, Y Nakamura
Vector
Construct size (kb): 4.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 2.299999952316284
Gene product: phosphorylase, glycogen; muscle (McArdle syndrome, glycogen storage disease type V) [PYGM]
Alleles: A1, A2, A3
Insert Size (kb)
2.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): AccI--4.8; BamHI--4.8; EcoRI--3.0, 1.75; HindIII--4.8; PvuII--2.4, 1.0, 0.85, 0.48.
Evidence of linkage (theta = 0.00, LOD score = 7.55) was found between the skeletal muscle glycogen phosphorylase (PYGM) gene on 11q and multiple endocrine neoplasia type 1 (MEN1).
Polymorphism also detected with TaqI.
Hybridization solution contained about 100 ug/ml total human DNA and 50% formamide.
Subcloned from cosmid cMCMP1 isolated by using the HM11 3' myophosphorylase cDNA.
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, RsaI.
References

Larsson C, et al. Multiple endocrine neoplasia type 1 gene maps to chromosome 11 and is lost in insulinoma. Nature 332: 85-87, 1988. PubMed: 2894610

Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence for human muscle glycogen phosphorylase (pMCMP1) on chromosome 11 [PYGM]. Nucleic Acids Res. 16: 10403, 1988. PubMed: 2461546

Nakamura Y, et al. Localization of the genetic defect in multiple endocrine neoplasia type I within a small region of chromosome 11. Am. J. Hum. Genet. 44: 751-755, 1989. PubMed: 2565085

Peter J O'Connell, personal communication

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