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pAS64-H4 plasmid in <em>E. coli</em> [full-length clone of tomato apical stunt viroid]
pAS64-H4 plasmid in <em>E. coli</em> [full-length clone of tomato apical stunt viroid]
規(guī)格:
貨期:
編號(hào):B219310
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pAS64-H4 plasmid in E. coli [full-length clone of tomato apical stunt viroid]
商品貨號(hào)
B219310
Designations
pAS64-H4 plasmid in E. coli [full-length clone of tomato apical stunt viroid]
Depositors
RA Owens
Vector
Total size of construct (kb): 3.36
Marker: ampR
Excise insert with: HindIII
Vector: pSP64
Vector type: plasmid
Vector size (kb): 3.0
Vector ends: HindIII
Markers: ampR
Insert
Insert size (kb): 0.36
Gene: Full-length Tomato Apical Stunt Viroid, TASV
DNA: cDNA
Strain: Ivory Coast
Insert end: HindIII
Insert Size (kb)
0.36
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
In E. coli containing the plasmid
Comments
The insert contains internal PstI, BamHI and SmaI sites. PstI digestion is diagnostic for the insert orientation.
Restriction digests of the clone give the following sizes (kb): HindIII--3.1, 0.36; PstI--3.2, 0.27; EcoRI--3.4.
Full-length (360 bp) cDNA fragment of TASV with HindIII ends was cloned into HindIII site of pSP64; noninfectious.
References

Kiefer MC, et al. Structural similarities between viroids and transposable genetic elements. Proc. Natl. Acad. Sci. USA 80: 6234-6238, 1983. PubMed: 6312450

Robert A Owens, personal communication

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