Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Animals were immunized with the synthetic peptide IGRGNFGEVFSG(C) corresponding to residues 567 through 578 of the FES sequence (formerly residues 529 through 540 of the HC/v-fes sequence).
Only the IgG2a immunoglobulin is reactive with the antigen.
In immunoblot assays, the antibody detects an 85 kd gag-fes fusion protein encoded by the Gardner-Arnstein strain of feline sarcoma in viral transformed mink lung cells. In an ELISA test, the antibody does not cross-react with the homologous region of src, yes, erb B, mil, raf, fms, fqr, abl, mos, CDC28 or the regulatory or catalytic subunits of bovine cyclic AMP dependent protein kinase. The IgG2a and IgG2b reactivities of the antibody with the fes peptide (567 to 578) were confirmed by ELISA. IgG2a was strongly positive while IgG2b was negative.
Only the IgG2a immunoglobulin is reactive with the antigen.
