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pHtk9 [KA27]
pHtk9 [KA27]
規格:
價格:
編號:B219987
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHtk9 [KA27]
商品貨號
B219987
Designations
pHtk9 [KA27]
Species
Homo sapiens, human
培養基
Depositors
KK Kidd
Applications
The 1.4 kb BamHI fragment has been used as a probe to detect RFLPs.
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.399999976158142
Gene product: thymidine kinase 1, soluble [TK1]
Alleles: A1, A2, B1, B2, B3
Insert Size (kb)
1.400
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XhoI--2.9, 1.6; HindIII--3.1, 1.5; PstI/PvuI--2.4, 1.2, 0.52; BamHI--3.0, 1.4; BglI--2.5, 1.2, 0.48.
The construction of this clone is not well documented. The 1.4 kb BamHI fragment has been used as a probe to detect RFLPs. It may also contain PstI/PvuII fragments of 0.4 and 0.5 kb.
Enzyme(s) not detecting polymorphism: ApaI, BamHI, BanII, BglI, BglII, CfoI, EcoRI, EcoRV, HincII, HinfI, HpaII, KpnI, MspI, PvuII, RsaI, SacI, ScaI, XbaI, XmnI (panel of 10-16 unrelated Caucasians).
References

Lin PF, et al. Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences. Mol. Cell. Biol. 5: 3149-3156, 1985. PubMed: 3018504

Murphy PD, et al. A frequent polymorphism for the cytosolic thymidine kinase gene, TK1, (17q21-q22) detected by the enzyme TaqI. Nucleic Acids Res. 14: 4381, 1986. PubMed: 3012471

Murphy PD, et al. A second useful polymorphism for the cytosolic thymidine kinase gene (TK1) with the enzyme BstEII which will allow haplotyping at this locus on chromosome 17 (q21-q22). Nucleic Acids Res. 15: 7212, 1987. PubMed: 2889186

Kenneth K Kidd, personal communication

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