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pEGsacIIRMC5 [NEB673]

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價格:

編號:B220467

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

pEGsacIIRMC5 [NEB673]

商品貨號

B220467

Designations

pEGsacIIRMC5 [NEB673]

Species

Streptomyces achromogenes Okami and Umezawa

培養基

Depositors

New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.

Applications

produces protein modification methylase SacII

produces protein restriction endonuclease SacII

Insert

DNA: genomic

Insert lengths(kb): 3.650000095367432

Gene product: restriction endonuclease SacII [hsdM]

Target Gene: modification methylase SacII, restriction endonuclease SacII

Insert Size (kb)

3.650

Media

ATCC^® Medium 1339: R2 YE medium

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Freeze dried S. lividans containing the plasmid

Comments

The insert contains the following restriction sites (approximate kb from the 5' end): ApaI--0.6, 2.1; BamHI--0.9, 3.0; BglII--2.2; NcoI--1.1, 1.8, 2.8; PstI--2.5.

The methylase gene is contained in the 2.5 kb HindIII/PstI fragment.

Verified to be viable in R2 YE medium + 50 ug/ml thiostrepton.

References

Guthrie EP, Meda MM. Method for producing and cloning SacII restriction endonuclease and methylase. US Patent 5,288,696 dated Feb 22 1994

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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