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pRH512
pRH512
規(guī)格:
價格:詢價
編號:B220958
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pRH512
商品貨號
B220958
Designations
pRH512
GenBank Number

M77182

培養(yǎng)基
Species
Amsacta moorei entomopoxvirus
Depositors
Univ. Florida Res. Fndn., Inc., RW Moyer, Univ. Florida Res. Fndn., Inc.
Applications
The Entomopoxvirus spheroidin and thymidine kinase genes make attractive expression cassettes for heterologous genes which can be used in vertebrate poxviruses such as vaccinia and swine pox virus.
Vector
Construct size (kb): 7.203999996185303
Insert
DNA: genomic
Insert lengths(kb): 4.504000186920166
Gene product: spheroidin
Insert Size (kb)
4.504
Media
ATCC® Medium 2000: 2X YT medium (1320)
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--4.5, 2.7; EcoRI--7.3; HindIII--6.4, 0.9.
AmEPV is one of the few insect poxviruses which replicate in insect cell lines. AmEPV is unable to replicate in vertebrate cell lines.
The Entomopoxvirus spheroidin and thymidine kinase genes make attractive expression cassettes for heterologous genes which can be used in vertebrate poxviruses such as vaccinia and swine pox virus.
Classification
Poxviridae, Entomopoxvirinae, Betaentomopoxvirus
References

Moyer RW, Hall RL. Entomopoxvirus spheroidin gene sequences. US Patent 5,476,781 dated Dec 19 1995

Hall RL, Moyer RW. Identification, cloning, and sequencing of a fragment of Amsacta moorei entomopoxvirus DNA containing the spheroidin gene and three vaccinia virus-related open reading frames. J. Virol. 65: 6516-6527, 1991. PubMed: 1942245

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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