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pEMBL18-Not (Sma-)

規格:

價格:

編號:B221108

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pEMBL18-Not (Sma-)

商品貨號

B221108

Designations

pEMBL18-Not (Sma-)

Depositors

A Craig

培養基

Biosafety Level

Vector Information

Size (kb): 3.9000000953674320

Vector: pEMBL18-Not (Sma-) (phagemid)

Promoters: Promoter lac

Construction: pEMBL18

Marker(s):ampR

Construct size (kb): 3.900000095367432

Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1, f1

Applications

vector for constructing genomic libraries

vector permitting construction of fusion proteins

vector permitting production of single-stranded DNA

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--3.9; NotI--4.0; EcoRI--3.9.

General purpose vector modified to include a NotI site to facilitate library construction and chromosome walking.

SmaI site in polylinker of pEMBL18 changed to a NotI site.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Ehrich E, et al. A family of cosmid vectors with the multi-copy R6K replication origin. Gene 57: 229-237, 1987. PubMed: 2961651

Shipped

freeze-dried

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