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pMS-TRP
pMS-TRP
規格:
價格:詢價
編號:B221479
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pMS-TRP
商品貨號
B221479
Designations
pMS-TRP
Depositors
SJ Elledge
培養基
Biosafety Level
1
Vector Information
Size (kb): 6.8280000686645510
Vector: pMS-TRP (phagemid)
Promoters: Promoter GAL
Marker(s):TRP1,ampR
Construct size (kb): 6.828000068664551
Features: marker(s): TRP1
marker(s): ampR
other: Myristoylation site
other: lox site
promoter: GAL
promoter: T7
replicon: 2 micron ori
replicon: f1
replicon: pMB1
MCS: EcoRI...SphI
restriction site: NotI
terminator: CYC1
epitope tag: 3x hemaglutinin (HA)
Applications
vector component of two-hybrid screening system
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.0, 2.8; BglI/XhoI--4.5, 2.3; SphI--6.8.
SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein.
Vector for use the the SOS Recruitment System (SRS). It encodes the v-Src myristoylation signal and can be used to construct expression libraries to be screened for interacting proteins.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808

Shipped
freeze-dried
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