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p52 [0488]

規格:

價格:

編號:B222383

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

p52 [0488]

商品貨號

B222383

Designations

p52 [0488]

GenBank Number

X13274

培養基

Species

Homo sapiens, human

Depositors

B Fennie

Vector

Construct size (kb): 5.599999904632568

Insert

DNA: cDNA

Insert lengths(kb): 0.8999999761581421

Tissue: lymphocyte, peripheral, SEB-treated

Gene product: interferon gamma [IFNG]

Insert Size (kb)

0.900

Media

ATCC^® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--5.6; BglI--2.4, 2.2, 0.8, 0.3; BglI/PvuI--2.4, 1.7, 0.8, 0.5, 0.3; HincII--4.0, 1.2, 0.45; PstI--4.8, 1.0.

The insert contains the following restriction sites (approximate kb from the 5' end): DraI--0.35, 0.36, 0.64; StyI--0.9.

The insert begins at about nt 250 of the sequence record and extends through the 3' end. Encodes the carboxy terminal 2/3 of the protein, and includes the 3' untranslated region.

References

Gray PW, et al. Expression of human immune interferon cDNA in E. coli and monkey cells. Nature 295: 503-508, 1982. PubMed: 6173769

Derynck R, et al. Human interferon gamma is encoded by a single class of mRNA. Nucleic Acids Res. 10: 3605-3615, 1982. PubMed: 6180381

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