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pIP82

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價格:

編號:B222499

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

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產品名稱

pIP82

商品貨號

B222499

Designations

pIP82

GenBank Number

X64810

培養基

Species

Homo sapiens, human

Applications

produces protein proprotein convertase subtilisin/kexin type 1 neuroendocrine convertase 1 (pro-protein-convertase PC1)

Vector

Construct size (kb): 6.099999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-3Zf(+)
Intact vector size: 3.199
Type of vector: phagemid
Vector end: XbaI
Vector end: XbaI
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII
PstI SphI HindIII-SP6
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PCSK1
Other gene symbols: NEC1
Genomic copy number: unique
Gene name: proprotein convertase subtilisin/kexin type 1
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q15-q21
Tissue: lung
Type of DNA: cDNA
Insert end: XbaI
Insert end: XbaI
Insert size (kb): 2.9
Cross references: DNA Seq. Acc.: X64810

Insert lengths(kb): 2.900000095367432

Tissue: lung

Gene product: proprotein convertase subtilisin/kexin type 1(neuroendocrine convertase 1 (pro-protein-convertase PC1)) [NEC1]

Target Gene: proprotein convertase subtilisin/kexin type 1

Insert Size (kb)

2.900

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--3.2, 2.9; BamHI--4.1, 2.0; EcoRI--3.5, 1.9, 0.65; PstI--3.3, 2.8; SacI--3.6, 2.6.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.12; EcoRI--1.82; BamHI--1.97.

The 3' XbaI site is not cleaved by XbaI. Sequence analysis indicates that the site is present.

Corresponds to nt 87-3039 of the sequence record. Contains the complete coding sequence (nt 190-2451 of the sequence record).

References

Creemers JW, et al. Expression in human lung tumor cells of the proprotein processing enzyme PC1/PC3. Cloning and primary sequence of a 5 kb cDNA. FEBS Lett. 300: 82-88, 1992. PubMed: 1547893

Wim J Van De Ven, personal communication

B238837：pIP82

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