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pUK4134

規格:

價格:詢價

編號:B222674

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

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產品名稱

pUK4134

商品貨號

B222674

Designations

pUK4134

Depositors

SC Straley

培養基

Biosafety Level

Vector Information

Size (kb): 7.0000000000000000

Vector: pUK4134 (plasmid)

Construction: pRTP1, pJM703.1

Marker(s):ampR,strS

Construct size (kb): 6.699999809265137

Features: marker(s): ampR
marker(s): strS
other: lambda cos site
replicon: R6K
replicon: oriT

Applications

contains sequence origin of transfer replication

contains sequence ribosomal protein S12

integrating vector

shuttle vector

vector permitting positive selection for integration

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--6.7, 0.5; EcoRI--6.4, 0.8; EcoRV--7.2; SalI--7.2.

RP6 is a suicide replicon in hosts not producing the pir protein.

RpsL (streptomycin sensitive) is dominant to streptomycin resistance, so loss of the plasmid can identified by streptomycin resistance and ampicillin sensitivity.

Suicide, gene replacement vector for Yersiniae and other gram-negative hosts that permits positive selection for loss of the plasmid after allelic exchange.

The vector also contains cos sites and oriT, and can be used for cosmid cloning or conjugative transfer.

The order of the major features in the plasmid is: EcoRV - R6K ori - oriT - SalI - cos - rpsL - ampR.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Skrzypek E, et al. New suicide vector for gene replacement in Yersiniae and other gram-negative bacteria. Plasmid 29: 160-163, 1993. PubMed: 8469722

Stibitz S, et al. The construction of a cloning vector designed for gene replacement in Bordetella pertussis. Gene 50: 133-140, 1986. PubMed: 2884169

Miller VL, Mekalanos JJ. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170: 2575-2583, 1988. PubMed: 2836362

Shipped

freeze-dried

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