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pTL-0059
pTL-0059
規格:
價格:詢價
編號:B222851
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pTL-0059
商品貨號
B222851
Designations
pTL-0059
Species
Tobacco etch virus
培養基
Depositors
WG Dougherty
Vector
Construct size (kb): 8.989999771118164
Insert
DNA: cDNA
Insert lengths(kb): 5.909999847412109
Gene product: VPg, 6 kDa
Insert Size (kb)
5.910
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.0; PstI--9.0; HindIII--5.2, 2.25, 1.1 + others; EcoRI--9.0.
The vector (pTL-8) allows in vitro expression of the insert as a fusion protein.
The insert includes TEV cDNA sequences from nt 0 to 5915.
Classification
Potyvirus
References

Allison R, et al. The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9-20, 1986.

Carrington JC, et al. Vectors for cell-free expression and mutagenesis of protein-coding sequences. Nucleic Acids Res. 15: 10066, 1987. PubMed: 3697076

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