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pBlueBac/PKCdelta
pBlueBac/PKCdelta
規(guī)格:
價(jià)格:
編號(hào):B223386
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pBlueBac/PKCdelta
商品貨號(hào)
B223386
Designations
pBlueBac/PKCdelta
GenBank Number

L07860

培養(yǎng)基
Species
Homo sapiens, human
Depositors
DJ Burns
Applications
in another host, produces protein protein kinase C, delta
Vector
Construct size (kb): 16.10000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBlueBac
Intact vector size: 14.000
Type of vector: phagemid
Vector end: NheI
Vector end: NheI
Cloning sites: NheI
Polylinker sites:
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
insert detection: lac
marker(s): ampR
promoter: polyhedrin
replicon: pMB1, f1, SV40
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCD
Gene name: protein kinase C, delta
Contains complete coding sequence?: Y
Localization: 3
Tissue: liver, HepG2 cell line
Type of DNA: cDNA
Insert end: Modification: NheI linkers
Insert end: Modification: NheI linkers
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: L07860
Insert lengths(kb): 2.099999904632568
Tissue: liver, HepG2 cell line
Gene product: protein kinase C, delta [PRKCD]
Target Gene: protein kinase C, delta
Insert Size (kb)
2.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): NheI--14.0, 2.1; BglI--5.6, 5.4, 2.2, 1.8, 1.2; HindIII--8.8, 4.0, 1.3, 1.1, 1.0; NcoI--14.0, 1.25, 0.5, 0.35; PstI--14.0, 1.1, 0.55, 0.3.
Insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.13; BglII--1.01; HindIII--0.85; NcoI--1.18, 1.64, 1.96; PstI--0.05, 0.33, 1.39; SalI--0.17.
Contains the complete coding sequence, and begins at the initiation codon (nt 59 of the sequence record).
References

Aris JP, et al. Molecular and biochemical characterization of a recombinant human PKC-delta family member. Biochim. Biophys. Acta 1174: 171-181, 1993. PubMed: 8357834

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