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pYAC4

規格:

價格:詢價

編號:B223672

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pYAC4

商品貨號

B223672

Designations

pYAC4

Depositors

MV Olson

培養基

U.S. Patent

4,889,806

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Biosafety Level

Vector Information

Size (kb): 11.4540004730224600

Vector: pYAC4 (YAC)

Marker(s): TRP1,URA3,ampR,HIS3,SUP4

Construct size (kb): 11.454

Features: marker(s): HIS3
marker(s): SUP4
marker(s): TRP1
marker(s): URA3
marker(s): ampR
other: telomere
replicon: ARS1
replicon: pMB1
centromere: CEN4

Applications

YL-type (YAC) shuttle vector

Shuttle vector

Vector for constructing genomic libraries

Comments

Yeast artificial chromosome (YAC) vector for cloning EcoRI fragments

A 0.7 kb BamHI-XhoI fragment contains two copies of the Tetrahymena thermophila macronuclear telomere

This yeast artificial chromosome contains ARS1, CEN4 and Tetrahymena telomeric elements

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37°C

References

Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825

Wells RA, et al. Telomere-related sequences at interstitial sites in the human genome. Genomics 8: 699-704, 1990. PubMed: 2276741

Burke DT, Olson MV. Preparation of clone libraries in yeast artificial-chromosome vectors. Methods Enzymol. 194: 251-270, 1991. PubMed: 2005791

Kuhn RM, Ludwig RA. Complete sequence of the yeast artificial chromosome cloning vector pYAC4. Gene 141: 125-127, 1994. PubMed: 8163163

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