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pTF7-3 plasmid in <em>Escherichia coli</em>
pTF7-3 plasmid in <em>Escherichia coli</em>
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B223997
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pTF7-3 plasmid in Escherichia coli
商品貨號(hào)
B223997
Designations
pTF7-3 plasmid in Escherichia coli
Species
Escherichia coli bacteriophage T7
培養(yǎng)基
Depositors
B Moss, National Institutes of Health
Applications
In another host, produces protein RNA polymerase
Vector
Construct information
Total size of construct (kb): unknown
Marker: ampR
Excise insert: BamHI

Vector information
Vector: pGS53
Vector type: plasmid
Vector size (kb): unknown
Vector ends: BamHI
Construction: pUC13 ; vaccinia Wyeth
Promoter: vaccinia p7.5
Replicon: pMB1
Markers: ampR
Insert
Insert size (kb): 2.65
Gene: gene 1, RNA polymerase
Genome: bacteriophage T7
DNA: genomic DNA
Contains complete coding sequence ?: Unknown
Insert ends: BamHI
Insert Size (kb)
2.650
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
ATCC VR-2153
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
In Escherichia coli containing the plasmid
Comments
Also available as the vaccinia recombinant, vTF7-3, ATCC® VR-2153™.
When this plasmid is transfected into vaccinia-virus infected cells, T7 RNA polymerase activity is expressed.
The vector pGS53 includes a P7.5 promoter with early and late regulatory signals upstream of BamHI and SmaI cloning sites. These are flanked by vaccinia TK sequences from the Wyeth strain to direct recombination with vaccinia virus.
References

Fuerst TR, et al. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83: 8122-8126, 1986. PubMed: 3095828

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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