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Avian reovirus type 3

規格:

價格:

編號:B224632

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Avian reovirus type 3

商品貨號

B224632

Classification

Reoviridae, Reovirus

Agent

Avian reovirus type 3

培養基

Strain

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation

Intestine from chicken with cloacal pasting

Product Format

frozen

Storage Conditions

?-70°C or colder

Comments

Deposited as Avian reovirus type 3. Pathogenic for newborn mice.

Effect on Host

CPE, syncytia formation

?CPE, syncytia formation

Recommended Host

Primary chicken embryo fibroblasts

Production Host: ?Primary CEF

Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 2 to 7 days in a humidified 5% CO₂ atmosphere

Name of Depositor

BL Patel, University of Minnesota, College of Veterinary Medicine

Source

Intestine from chicken with cloacal pasting

References

Deshmukh DR, Pomeroy BS. Avian reoviruses. I. Isolation and serological characterization. Avian Dis. 13: 239-243, 1969. PubMed: 4307737

B229404：Avian reovirus type 1

B228726：Rous associated virus-A (Avian leukosis-A)

B214447：Avian encephalomyelitis virus

B212624：Avian adenovirus Type 2

B204504：Avian myeloblastosis

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