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D-157

規(guī)格:

價格:詢價

編號:B224633

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

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產(chǎn)品名稱

D-157

商品貨號

B224633

Designations

D-157

GenBank Number

M85168

培養(yǎng)基

Species

Homo sapiens, human

Depositors

Y Chen

Vector

Construct size (kb): 4.760000228881836

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-3Zf(+)
Intact vector size: 3.199
Type of vector: phagemid
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII
PstI SphI HindIII-SP6
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AGL
Genomic copy number: unique
Gene name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen
debranching enzyme, glycogen storage disease type III)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 p21
Tissue: muscle
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.563
Cross references: DNA Seq. Acc.: M85168

Insert lengths(kb): 1.562999963760376

Tissue: muscle

Gene product: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) [AGL]

Insert Size (kb)

1.563

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 1.0, 0.5; HindIII--3.2, 1.1, 0.4; SacI/PstI--4.7; BamHI--4.0, 0.6; BglII--4.7; XbaI--4.55, 0.25.

The insert contains the following restriction sites (kb from the 5' end): BamHI--0.84; BglII--0.72; EcoRI--1.42; HindIII--0.39; NcoI--0.27, 0.36; XbaI--1.28.

Corresponds to nt 954-2490 of the published sequence, containing part of the coding region.

References

Yang BZ, et al. Molecular cloning and nucleotide sequence of cDNA encoding human muscle glycogen debranching enzyme. J. Biol. Chem. 267: 9294-9299, 1992. PubMed: 1374391

Yuan-Tsong Chen, personal communication

B195733：D-157

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