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k2.8
k2.8
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B224747
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
k2.8
商品貨號(hào)
B224747
Designations
k2.8
GenBank Number

L13974

培養(yǎng)基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NFE2
Gene name: nuclear factor (erythroid-derived 2), 45kD
Contains complete coding sequence?: U
Chromosome: 12; Localization: 12 q13
Tissue: K562 cells
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.496
Cross references: DNA Seq. Acc.: L13974
Nucleotides 1-1496 of the insert correspond to
nucleotides 1-1496 of L13974.
Insert lengths(kb): 1.496000051498413
Tissue: K562 cells
Gene product: nuclear factor (erythroid-derived 2), 45kD(nuclear factor 45) [NFE2]
Insert Size (kb)
1.496
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; EcoRI--2.7, 1.8; KpnI--3.3, 1.2; PstI--4.4; SalI--3.0, 1.3.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.10; BglI--0.21, 0.60, 0.65; KpnI--1.02; PstI--0.37; PvuII--0.30, 0.85, 1.13, 1.23; SacI--0.51; SmaI--0.06, 1.35; XbaI--0.20, 1.47.
References

Ney PA, et al. Purification of the human NF-E2 complex: cDNA cloning of the hematopoietic cell-specific subunit and evidence for an associated partner. Mol. Cell. Biol. 13: 5604-5612, 1993. PubMed: 8355703

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