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p58-CL39 [SGC35391, U24077]

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編號:B225558

品牌:Mingzhoubio

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RNA

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產(chǎn)品名稱

p58-CL39 [SGC35391, U24077]

商品貨號

B225558

Designations

p58-CL39 [SGC35391, U24077]

GenBank Number

U24077

培養(yǎng)基

Species

Homo sapiens, human

Depositors

N Wagtmann

Vector

Construct size (kb): 5.670000076293945

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSPORT1
Intact vector size: 4.109
Type of vector: plasmid
Vector end: NotI
Vector end: SalI
Cloning sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI
SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI
Sse8387I PstI
Polylinker sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI
SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI
Sse8387I PstI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: SP6, ->, 164-180
MCS: AatII...PstI, ->, 191-285
promoter for in vitro transcription: T7, <-, 298-318
insert detection: lacZ', <-, 1-341
repressor gene: lacI, <-, 464-1546
replicon: pMB1, 1903-1903
marker(s): ampR, <-, 2661-3521
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KIR
Other gene symbols: NKB1
Gene name: killer cell inhibitory receptor
ORF's seq. position: GDB probe: GDB:698358 ()
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.4; 19, 374.860000 cR
Tissue: Resting CD3- cells
Type of DNA: cDNA
Insert 5' end: Modification: SalI linker
Insert 3' end: Modification: NotI linker
Insert size (kb): 1.566
Cross references: DNA Seq. Acc.: U24077
Nucleotides 1-1532 of the insert correspond to
nucleotides 1-1532 of U24077.

Insert lengths(kb): 1.565999984741211

Tissue: Resting CD3- cells

Gene product: killer cell inhibitory receptor [KIR]

Insert Size (kb)

1.566

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): NotI/SalI--4.4, 1.6; EcoRV--5.7; PstI--5.0, 0.4, 0.35, 0.1; PvuII--3.8, 1.4, 0.6, 0.1; XhoI--5.7.

The insert contains the following restriction sites (approximate kb from the 5' end): BclI--0.35; PstI--0.04, 0.43, 0.51, 0.84; PvuII--1.12; SacI--0.45; StuI--1.05; XhoI--0.62.

Encodes a variant not recognized by either monoclonal antibody GL183 or EB6.

References

Wagtmann N, et al. Molecular clones of the p58NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains. Immunity 2: 439-449, 1995. PubMed: 7749980

B201288：p58-CL39 [SGC35391, U24077]

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