vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; KpnI--5.2; BamHI--5.2; PvuII--5.0, 0.3.
YC-type shuttle vector permitting visual detection of recombinants by lacZ alpha complementation and production of ssDNA (in the same sense as lacZ mRNA).
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604
