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plambdaP2 [pP2]
plambdaP2 [pP2]
規格:
價格:
編號:B226913
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
plambdaP2 [pP2]
商品貨號
B226913
Designations
plambdaP2 [pP2]
GenBank Number

J00290

培養基
Species
Homo sapiens, human
Depositors
SN Cohen
Applications
Enzyme(s) not detecting polymorphism: BglII, PstI, BamHI, AvaII, BglI, MspI. Tested against 20 unrelated individuals.
Vector
Construct size (kb): 6.300000190734863
Insert
DNA: genomic
Insert lengths(kb): 1.899999976158142
Tissue: liver
Gene product: proopiomelanocortin (adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin)(adrenocorticotropic hormone (ACTH)) [POMC]
Alleles: A1, A2, B1, B2
Insert Size (kb)
1.900
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.3; HindIII--6.3; PstI--4.4, 1.9.
The insert includes 5' flanking sequences and exon 1.
SstI polymorphism is about 10 kb 5' to exon 1 of POMC. Shows Mendelian segregation with codominance.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
Enzyme(s) not detecting polymorphism: BglII, PstI, BamHI, AvaII, BglI, MspI. Tested against 20 unrelated individuals.
References

Feder J, et al. A DNA polymorphism in close physical linkage with the proopimelanocortin gene. Am. J. Hum. Genet. 35: 1090-1096, 1983. PubMed: 6316780

Feder J, et al. DNA restriction fragment analysis of the proopiomelanocortin gene in schizophrenia and bipolar disorders. Am. J. Hum. Genet. 37: 286-294, 1985. PubMed: 2984925

Cochet M, et al. Characterization of the structural gene and putative 5'-regulatory sequences for human proopiomelanocortin. Nature 297: 335-339, 1982. PubMed: 6927850

Barbara Weiffenbach, personal communication

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