Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
Penicillin-Streptomycin-Amphotericin B Solution is used to reduce the chance of microbial contamination while propagating primary cells or continuous cell lines in culture.
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Product Format
frozen 1.0 mL
Storage Conditions
Store the Penicillin -Streptomycin -Amphotericin B Solution at -20°C or colder. When stored as directed, the product is stable until the expiration date on the label. It should be thawed only one time.
Comments
This antibiotic solution is formulated as follows: Penicillin 10,000 Units/mL, Streptomycin 10 mg/mL, Amphotericin B 25 µg/mL.
Complete Growth Medium
Take the vial from storage and thaw. Mix well or vortex briefly to make sure the solution is homogenous.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the appropriate volume of the antibiotic/antimycotic solution to complete media.
Dilute 1:1000 when using to supplement complete growth media; i.e., 0.5 mL of Penicillin-Streptomycin-Amphotericin B Solution per 500 mL of complete cell-specific media.
Dilute 1:100 when using during cell isolation procedures; i.e., 0.5 mL of Penicillin-Streptomycin-Amphotericin B Solution per 50 mL of complete cell-specific growth media.
Tightly cap the bottle (or other flask) of supplemented growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the flask.
Fully supplemented media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, fully supplemented media is stable for 30 days.
Volume
1.0 mL
Sterility Tests
Negative for bacteria, fungi and yeast.
Functional Tests
Each lot is assessed for acceptable growth of human primary cells compared to control. A Certificate of Analysis (COA) is available upon request for each lot of Penicillin-Streptomycin-Amphotericin B Solution.
