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pBShUBC9(S)

規(guī)格:

價格:

編號:B227143

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pBShUBC9(S)

商品貨號

B227143

Designations

pBShUBC9(S)

GenBank Number

U66818

培養(yǎng)基

Species

Homo sapiens, human

Depositors

C Wang

Applications

contains sequence ubiquitin-conjugating enzyme E2I

Vector

Construct size (kb): 4.050000190734863

Insert

DNA: cDNA

Insert lengths(kb): 1.100000023841858

Tissue: placenta

Gene product: ubiquitin-conjugating enzyme E2I [UBC9]

Target Gene: ubiquitin-conjugating enzyme E2I

Insert Size (kb)

1.100

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.1; PvuII--2.6, 1.0, 0.45; XbaI--3.1, 0.88.

The insert contains the following restriction sites (approximate kb from the 5' end): NotI--0.06; AvaI--0.07; StuI--0.48; PvuII--0.94; XbaI--0.98.

ATCC 99599 and ATCC 99600 are the same for the initial 15 bp upstream of the initiation codon, the CDS, and the 3' UTR. The clones differ at the 5' end of the 5' UTR. ATCC 99599 contains a 791 bp sequence while ATCC 99600 has a different 72 bp sequence.

References

Wang ZY, et al. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J. Biol. Chem. 271: 24811-24816, 1996. PubMed: 8798754

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