This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Restriction digests of the clone give the following sizes (kb): BamHI--6.9; ClaI--6.9; EcoRI--4.4, 2.3; EcoRV--4.6, 2.4; HindIII--6.9; SalI--6.9.
A map is provided in this reference.
Shuttle vector that can replicate and be selected in Escherichia coli and mammalian cells.
The order of the major features in this plasmid is: EcoRI - ClaI - HindIII - BamHI - SalI - oriP - HSV-1 tk polyadenylation - <- hygR - HSV-1 tk promoter - < - ampR.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Sugden WM. Recombinant vector and eukaryotic host transformed thereby. US Patent 4,686,186 dated Aug 11 1987
. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: CSHL Press; 1989.
