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當前位置: 首頁 > ATCC代理 > cHuVim1 [cHuVIM1] B227442
cHuVim1 [cHuVIM1]
cHuVim1 [cHuVIM1]
規格:
價格:
編號:B227442
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
cHuVim1 [cHuVIM1]
商品貨號
B227442
Designations
cHuVim1 [cHuVIM1]
Species
Homo sapiens, human
培養基
Depositors
D Paulin
Applications
Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences.
cHuVim1 has been used to detect a 2.0 kb mRNA in various mouse cell lines.
Vector
Construct size (kb): 3.799999952316284
Insert
DNA: cDNA
Insert lengths(kb): 1.100000023841858
Tissue: fibroblast
Gene product: vimentin [VIM]
Alleles: A1, A2
Insert Size (kb)
1.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Insert contains the following sites separated by (bp) (approx): EcoRI- 130- MspI- 120- MspI- 35- HincII- 95- PstI- 115- PstI- 100- MspI- 140- PstI- 140- MspI- 170- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 1.2; HindIII--4.0; PstI--3.1, 0.46, 0.29; BamHI--3.8; SalI--4.0.
Clone starts at nt 331 (corresponding to amino acid 111) after the ATG and extends to the stop codon. Includes part of exon 1 through exon 9.
Shows 90% homology with coding region of hamster vimentin. Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences.
cHuVim1 has been used to detect a 2.0 kb mRNA in various mouse cell lines.
References

Perreau J, et al. Nucleotide sequence of the human vimentin gene and regulation of its transcription in tissues and cultured cells. Gene 62: 7-16, 1988. PubMed: 3371665

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