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p3
p3
規格:
價格:
編號:B227578
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p3
商品貨號
B227578
Designations
p3
GenBank Number

M73969

培養基
Species
Homo sapiens, human
Depositors
PM Murphy
Applications
A 1.195 kb BglII/HindII fragment contains the complete coding sequence and has been used as a probe.
Vector
Construct size (kb): 4.599999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IL8RB
Gene name: interleukin 8 receptor, beta
Contains complete coding sequence?: Y
Chromosome: 2; Localization: 2 q35
Tissue: HL-60 cell line
Disease: promyelocytic leukemia
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M73969
Nucleotides 165-1675 of the insert correspond to
nucleotides 1-1510 of M73969.
Insert lengths(kb): 1.700000047683716
Tissue: HL-60 cell line promyelocytic leukemia
Disease: promyelocytic leukemia
Gene product: interleukin 8 receptor, beta [IL8RB]
Insert Size (kb)
1.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.7; XhoI--4.7; EcoRI/XhoI--2.9, 1.8; PstI--4.7; BglII/HindIII--3.5, 1.2.
Includes 156 nt in addition to the 5' untranslated region reported in GenBank M73969. Nucleotides 1-9 of the insert are linker.
A 1.195 kb BglII/HindII fragment contains the complete coding sequence and has been used as a probe.
References

Murphy PM, Tiffany HL. Cloning of complementary DNA encoding a functional human interleukin-8 receptor. Science 253: 1280-1283, 1991. PubMed: 1891716

Ahuja SK, et al. Molecular evolution of the human interleukin-8 receptor gene cluster. Nat. Genet. 2: 31-36, 1992. PubMed: 1303245

Ahuja SK, Murphy PM. Molecular piracy of mammalian interleukin-8 receptor type B by Herpesvirus Saimiri. J. Biol. Chem. 268: 20691-20694, 1993. PubMed: 8407886

Ahuja SK, et al. Comparison of the genomic organization and promoter function for human interleukin-8 receptors A and B. J. Biol. Chem. 269: 26381-26389, 1994. PubMed: 7929358

Ake Lernmark, personal communication

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