蜜臀久久99精品久久久久久,国产精品熟女久久久久久,免费黄色三级

pLDLR-2

規(guī)格:

價格:

編號:B227662

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

pLDLR-2

商品貨號

B227662

Designations

pLDLR-2

Species

Homo sapiens, human

培養(yǎng)基

Depositors

DW Russell

Applications

in another host, produces protein low density lipoprotein receptor (familial hypercholesterolemia) low density lipoprotein receptor

Vector

Construct size (kb): 8.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: LDLR
Genomic copy number: unique
Gene name: low density lipoprotein receptor (familial
hypercholesterolemia)
Contains complete coding sequence?: U
Chromosome: 19; Localization: 19 p13.2
Tissue: fetal adrenal gland
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 5.3
Cross references:

Insert lengths(kb): 5.300000190734863

Tissue: fetal adrenal gland

Gene product: low density lipoprotein receptor (familial hypercholesterolemia)(low density lipoprotein receptor) [LDLR]

Alleles: A2, E1, E2, A1, A2, B1, B2, D1, D2, K1, K2, Q1, Q2, S2, A1, S1

Target Gene: low density lipoprotein receptor (familial hypercholesterolemia)

Insert Size (kb)

5.300

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): PstI--3.0, 1.8, 1.4, 1.1, 0.8; xbaI--uncut; BamHI--3.0, 1.9, 1.7, 1.4, 0.32; HindIII--8.0; EcoRI--8.0.

Encodes nt 1-2580 of the published sequence, including the entire coding region and 3' untranslated region and a portion of the 5' untranslated region.

When pLDLR-2 is transfected into COS-M6 cells, protein is expressed that binds low-density lipoprotein and that is recognized by monoclonal antibody IgG-C7, specific to the human receptor.

The vector contains a portion of pBR322 and SV40 sequences including SV40 ori, 16S and 19S donor and acceptor splicing sites, and polyadenylation signals.

Constructed by joining the cDNA insert of p203 (nt 1-323) and p101 (nt 324-5144) via overlapping HgiAI sites.

References

Yamamoto T, et al. The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell 39: 27-38, 1984. PubMed: 6091915

Brown MS, et al. Methods and compositions for the detection of familial hypercholesterolemia. US Patent 4,966,837 dated Oct 30 1990

Sudhof TC, et al. The LDL receptor gene: a mosaic of exons shared with different proteins. Science 228: 815-822, 1985. PubMed: 2988123

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479