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pSNAG

規(guī)格:

價格:

編號:B227679

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

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產(chǎn)品名稱

pSNAG

商品貨號

B227679

Designations

pSNAG

Depositors

C Hovens

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 5.1960000991821290

Vector: pSNAG (phagemid)

Promoters: Promoter CMV

Construction: pSCTEV, pSuperVSV

Marker(s):ampR

Construct size (kb): 5.196000099182129

Features: marker(s): ampR
other: Gal4p DNA-binding domain (DBD)
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: SfuI...XbaI
terminator: SV40
epitope tag: VSV-G

Applications

expression vector

vector permitting construction of fusion proteins

vector permitting production of single-stranded DNA

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2.

The vector was constructed as follows: pSCTEV Gal4p/VP16 digested with SphI and KpnI and ligated to adaptors was cloned into a HindIII/BamHI cut pSuperVSV vector.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Buchert M, et al. Useful vectors for the two-hybrid system in mammalian cells. BioTechniques 23: 396-402, 1997. PubMed: 9298205