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pMMB277
pMMB277
規(guī)格:
價(jià)格:
編號(hào):B227690
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMMB277
商品貨號(hào)
B227690
Designations
pMMB277
Depositors
VM Morales
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 9.2370004653930660
Vector: pMMB277 (plasmid)
Promoters: Promoter tac
Construction: pMMB206, pMMB311
Marker(s):cmlR
Construct size (kb): 9.237000465393066
Features: insert detection: lacZ'
marker(s): cmlR
promoter: tac
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; EcoRV--4.2, 1.7, 1.5, 1.1, 0.9; EcoRI/MluI--8.4, 0.8.
Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation.
Constructed by replacing the MluI/EcoRI fragment of pMMB206 (taclacUV5 promoter) with the MluI/EcoRI fragment from pMMB311 (tac promoter).
Media
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References

Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347

Shipped
freeze-dried
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