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pMMB277

規格:

價格:詢價

編號:B227690

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pMMB277

商品貨號

B227690

Designations

pMMB277

Depositors

VM Morales

培養基

Biosafety Level

Vector Information

Size (kb): 9.2370004653930660

Vector: pMMB277 (plasmid)

Promoters: Promoter tac

Construction: pMMB206, pMMB311

Marker(s):cmlR

Construct size (kb): 9.237000465393066

Features: insert detection: lacZ'
marker(s): cmlR
promoter: tac
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB

Applications

expression vector

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; EcoRV--4.2, 1.7, 1.5, 1.1, 0.9; EcoRI/MluI--8.4, 0.8.

Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation.

Constructed by replacing the MluI/EcoRI fragment of pMMB206 (taclacUV5 promoter) with the MluI/EcoRI fragment from pMMB311 (tac promoter).

Media

ATCC^® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol

Growth Conditions

Temperature: 37.0°C

References

Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347

Shipped

freeze-dried

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