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lambdaSP14

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價格:

編號:B227755

品牌:Mingzhoubio

標準菌株

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DNA

RNA

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凍干粉

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甘油

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產品名稱

lambdaSP14

商品貨號

B227755

Designations

lambdaSP14

Species

Homo sapiens, human

培養基

Depositors

M Lefranc

Vector

Construct size (kb): 44.70000076293945

Insert

DNA: genomic

Insert lengths(kb): 15.19999980926514

Tissue: T-lymphocyte SP-F7 cell line

Gene product: T cell receptor, gamma, variable region V3 [TCRGJP]

Alleles: A2, A1

Insert Size (kb)

15.200

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried bacteria-free lysate

Comments

Restriction digests of the clone give the following sizes (kb): SmaI--18.0, .5, 7.0, 6.2, 5.0; BamHI--20.0, 12.0, 9.0, 3.6; EcoRI--21.0, 12.0, 5.8, 2.3, 1.9, 1.3; XbaI--20.0, 15.0, 9.5;

PvuI--22.0, 15.0, 7.5; SacI--19.0 9.5, 6.5, 4.2, 4.0.

The insert contains the following restriction sites (approximate kb from the5' end): EcoRI--1.4, 3.2, 9.1, 10.7; 11.9; 13.8; HindIII--1.3, 5.8, 6.4, 8.3, 9.9, 12.1, 14.0; KpnI--3.4, 3.9, 8.1.

Contains a non-functional V9-JP rearrangement generating a frameshift in the N diversity region. Also contains germline V2 and an unusual reqrrangement abutting V3 to J1 recombination signals in an inverted orientation.

lambdaSP14 (ATCC 65836, 65837) and lambdaSP19 (ATCC 65838, 65839) are end clones from the same inversion (47 kb) event.

References

Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738

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