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HFBCB31
HFBCB31
規(guī)格:
價(jià)格:
編號(hào):B227812
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
HFBCB31
商品貨號(hào)
B227812
Designations
HFBCB31
GenBank Number

M78393

培養(yǎng)基
Species
Homo sapiens, human
Applications
Expected product size (bp): 150. Observed product sizes (bp): 152 (human).
Vector
Construct size (kb): 5.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GST*
Gene name: glutathione S-transferase
Contains complete coding sequence?: U
Chromosome: 21
Genome: Homo sapiens
Gene symbol: D21S363E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: 21; Localization: 21
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.4
Cross references: DNA Seq. Acc.: M78393; EST: EST00541; Primer: M13 Forward
Insert lengths(kb): 2.400000095367432
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: glutathione S-transferase [GST*]
Insert Size (kb)
2.400
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.3, 1.1; PstI--5.4; HindIII--3.6, 1.8.
EST00541 can be detected in genomic DNA using the following oligonucleotides (5'-3'): CCGTCGGTAACAGAAAACTC and ACACAGTCCTGTAATACCCTCC.
The 1.1 kb fragment contains EST00541. The 1.3 kb fragment does not map to chromosome 21 and contains sequences homologous to glutathione S-transferase.
Expected product size (bp): 150. Observed product sizes (bp): 152 (human).
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Durkin AS, et al. Chromosomal assignment of 39 human brain expressed sequence tags (ESTs) by analyzing fluorescently-labeled PCR products from hybrid cell panels. Genomics 14: 808-810, 1992. PubMed: 1427913

Maglott DR, et al259 human brain expressed sequence tags (ESTs): chromosome localization, subregional assignment, and sequence analysisIn: Maglott DR, et alIdentification of transcribed sequencesNew YorkPlenum Presspp. 273-288, 1994

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