ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
When excising insert by EcoRI/BglI, the vector is cut in 2, and the insert, which is the largest band, has about 150 bp of pUC18.
Restriction digests of the clone give the following sizes (kb): BglI--4.1, 1.15; BamHI--5.2; EcoRI--5.2; SalI--5.2; EcoRI/BglI--2.85, 1.35, 1.05; EcoRI/HindIII--2.7, 2.7.
Enzyme(s) not detecting polymorphism: MspI, TaqI, EcoRI, BamHI (in a limited study).
