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當(dāng)前位置: 首頁 > ATCC代理 > clone 3.10 B227986
clone 3.10
clone 3.10
規(guī)格:
價(jià)格:
編號:B227986
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
clone 3.10
商品貨號
B227986
Designations
clone 3.10
GenBank Number

M59911

培養(yǎng)基
Species
Homo sapiens, human
Depositors
Q Yuan, ME Hemler
Vector
Construct size (kb): 6.730000019073486
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: XbaI closer to SacI
Vector end: XbaI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ITGA3
Gene name: integrin, alpha 3 (CD49C, alpha 3 subunit of VLA-3
receptor)
Contains complete coding sequence?: Y
Tissue: endothelium cells and HepG2 cell line
Type of DNA: cDNA
Insert end: Modification: XbaI linker
Insert end: Modification: SalI linker
Insert size (kb): 3.47
Cross references: DNA Seq. Acc.: M59911
Nucleotides 1-3470 of the insert correspond to
nucleotides 1-3470 of M59911.
Insert lengths(kb): 3.47000002861023
Tissue: endothelium cells and HepG2 cell line
Gene product: integrin, alpha 3 (CD49C, alpha 3 subunit of VLA-3 receptor)(integrin, alpha 3 subunit) [ITGA3]
Insert Size (kb)
3.470
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--3.5, 3.0; HindIII--6.5; EcoRI--6.5; BamHI--3.1, 2.9, 0.5; NdeI--6.5.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--2.39; EcoRI--2.47; BamHI--2.7, 3.32.
Constructed from clones 3.410, 3.285 and 3.520.
References

Takada Y, et al. Molecular cloning and expression of the cDNA for alpha 3 subunit of human alpha 3 beta 1 (VLA-3), an integrin receptor for fibronectin, laminin, and collagen. J. Cell Biol. 115: 257-266, 1991. PubMed: 1655803

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