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HD2G1
HD2G1
規格:
價格:
編號:B228014
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HD2G1
商品貨號
B228014
Designations
HD2G1
GenBank Number

M29066

培養基
Species
Homo sapiens, human
Depositors
O Civelli, DK Grandy
Applications
Codominant inheritance was observed in 3 informative families (39 children).
Vector
Construct size (kb): 44.0
Insert
DNA: genomic
Insert lengths(kb): 16.0
Tissue: male
Gene product: dopamine receptor D2 [DRD2]
Alleles: A1, B1, B2, A2
Insert Size (kb)
16.0
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 9.0, 5.0, 4.3, 1.8, 1.7, 1.7, 0.7; BglII--25.0, 7.0, 3.2, 3.0, 2.4, 1.5, 0.68; PvuI--20.0, 12.0, 8.0; HindIII--21.0, 18.0, 4.3, 0.8; SmaI--20.0, 19.0, 5.6.
The insert contains the following sites separated by (kb)(approx): SalI, MboII- 1.5- BamHI- 1.7- BamHI- 1.7- BamHI- 4.3- BamHI- 1.8- BamHI- 0.65- BamHI- 5.0- SmaI- 0.6- BamHI- 0.7- MboII, SalI.
Codominant inheritance was observed in 3 informative families (39 children).
The 1.6 kb BamHI fragment contains exon 7 and 3' sequences. Exon 7, which has been sequenced, encodes the last 64 amino acids and includes 1.2 kb of 3' non-coding sequence.
The BamHI 1.6 doublet detects the TaqI RFLP associated with DRD2.
Use standard hybridization conditions with the addition of human placental DNA to block repeat sequences. Washing conditions are standard with a final wash at 0.1 x SSC, 0.1% SDS, 65C.
Enzyme(s) not detecting polymorphism: 31 enzymes.
References

Grandy DK, et al. The human dopamine D2 receptor gene is located on chromosome 11 at q22-q23 and identifies a TaqI RFLP. Am. J. Hum. Genet. 45: 778-785, 1989. PubMed: 2573278

Grandy DK, et al. Cloning of the cDNA and gene for a human D2 dopamine receptor. Proc. Natl. Acad. Sci. USA 86: 9762-9766, 1989. PubMed: 2532362

Olivier Civelli, personal communication

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