最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > alpha1JN [alphaNJ, pJN I] B228432
alpha1JN [alphaNJ, pJN I]
alpha1JN [alphaNJ, pJN I]
規格:
價格:
編號:B228432
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
alpha1JN [alphaNJ, pJN I]
商品貨號
B228432
Designations
alpha1JN [alphaNJ, pJN I]
GenBank Number

J00067

培養基
Species
Homo sapiens, human
Depositors
NA Kalsheker
Applications
Enzyme(s) not detecting polymorphism (for alpha1-antitrypsin gene-related sequence): BamHI, HindIII, MspI, PstI, XbaI, XhoII (tested against 20 unrelated Caucasians).
Vector
Construct size (kb): 4.0
Insert
DNA: cDNA
Insert lengths(kb): 0.25
Tissue: liver
Gene product: alpha-1-antitrypsin-like [PI]
Alleles: G3, A1, A2, B1, B2, D2, G1, G2, J2, N1, N2, C1, C2, D1, J1, A1, A2, D1, D2
Insert Size (kb)
0.25
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/AvaI--2.55, 1.45; PstI--4.0; EcoRI--4.0; AvaI--4.0; HindIII--4.0.
The sequence starts at amino acid 363 and continues through a poly(A) tail of 50 bp. The PstI site at the 3' end was not reconstituted.
The insert encompasses the last exon including the 3' untranslated region.
Codominant segregation for the EcoRI RFLP has been demonstrated in 2 informative families.
The gene-related sequence which demonstrates the EcoRI polymorphism maps 15 kb downstream from the alpha-1-antitrypsin gene.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
The clone is for alpha1-antitrypsin but can detect a related sequence. Signals can be distinguished by the sequence and the sizes of fragments generated. At higher stringency this can be made specific for alpha1-antitrypsin.
Enzyme(s) not detecting polymorphism (for alpha1-antitrypsin gene-related sequence): BamHI, HindIII, MspI, PstI, XbaI, XhoII (tested against 20 unrelated Caucasians).
References

Rogers J, et al. The isolation of a clone for human alpha 1-antitrypsin and the detection of alpha 1-antitrypsin in mRNA from liver and leukocytes. Biochem. Biophys. Res. Commun. 116: 375-382, 1983. PubMed: 6606425

Hodgson I, Kalsheker N. RFLP for a gene-related sequence of alpha 1-antitrypsin (AAT). Nucleic Acids Res. 14: 6779, 1986. PubMed: 3018681

Barbara Weiffenbach, personal communication

Noor A Kalsheker, personal communication

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479